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本研究以低茎秆含糖量的粒用高粱品系LR625(P1)和高茎秆含糖量的甜高粱品系Rio(P2)杂交获得的234个F2:3家系为试验材料,构建了含有92个高粱SSR标记、6个玉米SSR标记和自主开发的28个INDEL标记的遗传连锁图谱,并进行了茎汁混合锤度、茎秆重和出汁率性状的QTL定位。研究结果显示:共检测到7个与茎汁混合锤度有关的QTL,分布在高粱的第LG-3、LG-5、LG-7和LG-9连锁群上,解释的总表型变异为62.45%;4个与茎秆重有关的QTL,分布在高粱的第LG-2、LG-5、LG-6和LG-7连锁群上,解释的总表型变异为60.92%;8个与出汁率有关的QTL,分布在高粱的第LG-1、LG-2、LG-3、LG-6、LG-7和LG-9连锁群上,解释的总表型变异为99.75%。研究结果有助于深入理解茎秆含糖量遗传基础,为茎秆含糖量QTL精细定位和基因挖掘提供依据。
In this study, 234 F2: 3 pedigrees obtained from crossing sweet sorghum line LR625 (P1) with high stalk sugar content and sweet sorghum line Rio (P2) Sorghum SSR markers, six maize SSR markers and 28 INDEL markers independently developed. The QTL mapping of mixed juice, stem weight and juice yield of stem juice was conducted. The results showed that seven QTLs related to the mixed brix of stem juice were detected in LG-3, LG-5, LG-7 and LG-9 linkage groups in sorghum, and the total phenotypic variance explained 62.45%, respectively. Four QTLs related to stem weight were distributed on linkage groups LG-2, LG-5, LG-6 and LG-7 in sorghum, explaining a total phenotypic variation of 60.92% The QTLs related to juicing rate were distributed on linkage groups LG-1, LG-2, LG-3, LG-6, LG-7 and LG-9 in sorghum, with a total phenotypic variation of 99.75%. The results are helpful to understand the genetic basis of sugar content in stems and provide basis for fine mapping and gene digging of QTL for sugar content in stems.