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转基因植物的遗传稳定性分析是基因工程的重要环节。本研究以转基因白桦的控制授粉杂交子代家系、正反交子代家系以及自由授粉的半同胞子代家系为研究对象,测定了各家系间的种子千粒质量、发芽率、发芽势以及活力指数。结果表明:各家系间种子千粒质量及种子活力等指标差异达到极显著水平(P<0.01),各性状的变异系数在41.09%~73.16%之间,遗传力在98.00%以上;种子千粒质量与发芽率、发芽势、活力指数呈极显著正相关,相关系数分别为0.916、0.803与0.896。转基因株系与非转基因株系正反交群体的外源基因检测表明:外源基因通过雌配子的传递效率高于雄配子,雌、雄配子外源基因传递效率分别在60.00%、30.00%左右。杂交子代家系外源基因检测结果表明,4个转基因株系有1个插入位点,1个株系有2个插入位点。卡方检验表明:外源基因插入位点为一个的转基因株系,其配子类型符合1∶1的分离规律;外源基因插入位点为2个转基因株系,其配子类型符合3∶1的分离规律。
Genetic stability analysis of transgenic plants is an important part of genetic engineering. In this study, transgenic birch control of pollination hybrid progeny, bovine progeny crossbred families and free pollinated half sibling progeny as the object of study, the determination of the seed quality, germination rate, germination potential, Vitality index. The results showed that there was significant difference (P <0.01) in seed quality and seed vigor among all the families, the coefficient of variation of each trait ranged from 41.09% to 73.16% and the heritability over 98.00% There was a significant positive correlation between grain quality and germination rate, germination potential and vigor index, with correlation coefficients of 0.916, 0.803 and 0.896 respectively. The exogenous gene detection of the reciprocal cross between transgenic and non-transgenic lines showed that the efficiency of exogenous gene transfer through female gametes was higher than that of male gametes and that of female and male gametes was 60.00% and 30.00% respectively. The results of heterologous gene test in hybrid progeny showed that there were 1 locus in 4 transgenic lines and 2 loci in 1 locus. The chi-square test showed that the exogenous gene insertion site was a transgenic line whose gamete type was in accordance with the rule of 1: 1 segregation. The exogenous gene insertion site was 2 transgenic lines with the gamete type of 3: 1 Separation rules.