论文部分内容阅读
人 Ｂ１结合因子（ｈｕｍａｎ Ｂ１ ｂｉｎｄｉｎｇ ｆａｃｔｏｒ， ｈＢ１Ｆ）是本实验室克隆到的一个新的转录因子，它能够结合于ＨＢＶ增强子Ⅱ（ＥＮ Ⅱ）的Ｂ１区并反式激活其功能．对ｈＢ１Ｆ和其他反式激活ＥＮ Ⅱ的肝细胞核因子间在功能上的相互关系进行了研究．氯霉素乙酰转移酶（ｃｈｌｏｒａｍｐｈｅｎｉｃｏｌａｃｅｔｙｌｔｒａｎｓｆｅｒａｓｅ， ＣＡＴ）报告基因分析发现， ｈＢ１Ｆ与 ＨＮＦ３β， ＨＮＦ４间在激活 ＥＮ Ⅱ的功能上仅存在简单的加和性，而ｈＢ１Ｆ和ＨＮＦ１共同存在的情况下却能够产生很强的协同效应．进一步利用ＧＳＴｐｕｌｌ－ｄｏｗｎ实验在体外证明了这两种因子间存在特异的直接相互作用．研究结果表明，ｈＢ１Ｆ与ＨＮＦ１协同地调控ＨＢＶ增强子Ⅱ的功能．
Human B1 binding factor (hB1F) is a new transcription factor cloned in our laboratory. It can bind to the B1 region of HBV enhancer Ⅱ (EN Ⅱ) and transactivate its function. The functional relationship between hB1F and other trans-activated EN II hepatocytes was studied. Chloramphenicolacetyltransferase (CAT) reporter gene analysis revealed that there was only simple additive function between hB1F and HNF3β and HNF4 in the activation of EN Ⅱ, but the combination of hB1F and HNF1 could produce very good Strong synergy. Further use of GSTpull-down experiments in vitro proved that there is a specific direct interaction between these two factors. The results show that hB1F and HNF1 synergistically regulate the function of HBV enhancer Ⅱ.