本文根据基本方程Ink′=b_0+b_1cB+b_2IncB以及色谱柱的总分高效能指标K_1,计算出多元组份分离时所需的最小相对保留值a_(min)和最难分离物质对的相对保留值a′.由a′必须大于′a_(min)以及最后流出组份k′_(lasl)最小的最佳冲洗剂组成选择原则,建立起以最少的实验数据快速进行最佳冲洗剂组成选择的方法.文中给出了以甲醇、乙醚、已烷作三元冲洗剂,以5对氨基酸作样品,进行最佳冲洗剂组成选择的例子,色谱图表明:理论预测与实验结果吻合。 Based on the basic equation Ink ’= b_0 + b_1cB + b_2IncB and the column’s high performance index K_1, the minimum relative retention (a min) and the relative retention of the most difficult pairs The best rinse agent composition selection principle, with a ’must be greater than’ a_ (min) and minimum last outflow component k’_ (lasl), establishes the optimum rinse agent composition selection with minimal experimental data The method for selecting the optimal composition of rinsing agent with methanol, diethyl ether and hexane as the ternary rinsing agent and 5 pairs of amino acids as samples was given. The chromatogram shows that the theoretical prediction agrees well with the experimental results.