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目的研究姜黄素对人肺成纤维细胞(human lung fibroblasts,HLFs)体外生长、细胞周期及凋亡的影响。方法应用CCK-8、Annexin-V/PI、PI流式细胞分析术、免疫印迹法,检测姜黄素对细胞的增殖、凋亡、细胞周期和Bax蛋白表达的影响。结果姜黄素对HLFs的增殖有抑制作用,可促进HLFs的早期凋亡,对细胞周期可产生G2/M期阻滞,增加HLFs的Bax蛋白的表达。结论姜黄素可抑制HLFs的增殖并能诱导其凋亡,其机制可能与细胞的G2/M期阻滞及凋亡相关蛋白的表达增强有关。
Objective To study the effects of curcumin on human lung fibroblasts (HLFs) growth, cell cycle and apoptosis in vitro. Methods The effects of curcumin on cell proliferation, apoptosis, cell cycle and Bax protein expression were detected by CCK-8, Annexin-V / PI, PI flow cytometry and Western blotting. Results Curcumin inhibited the proliferation of HLFs, promoted the early apoptosis of HLFs, blocked G2 / M phase of the cell cycle and increased the expression of Bax protein in HLFs. Conclusion Curcumin can inhibit the proliferation of HLFs and induce their apoptosis. The mechanism may be related to the G2 / M arrest and the expression of apoptosis-related proteins.