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雌性大鼠,体重200—250g,按Yaksh和Rudy方法作脊髓蛛网膜下膣(ith)慢性套管。术后24h开始实验。部分动物作侧脑室(ICV)插管,用牙科水泥固定于颅骨。实验在术后一周进行。用辐射热测大鼠甩尾潜伏期(TFL)作为痛阈。注射药物以连续累加剂量给予,即每隔一定时间(10或30min)注射药物一次,后一次的剂量为前一次的一倍。ith注射量每次10μl,侧脑室注射量每次12μl。实验前测基础痛阈,注药后每隔10min测痛一次。将所测痛阈与基础痛阈相比,用变化百分数表示。
Female rats weighing 200-250 g were chronically cannulated with subarachnoid ith (ith) according to the Yaksh and Rudy methods. 24h after the start of the experiment. Some animals for lateral ventricle (ICV) intubation, with dental cement fixed to the skull. The experiment was performed one week after surgery. Rat tail flick latency (TFL) was measured as the pain threshold with radiant heat. Injectables are given in consecutive cumulative doses, ie once at regular intervals (10 or 30 minutes) and once after the previous dose. ith injection volume of 10μl, intraventricular injection volume of 12μl each time. The basis of pre-test pain threshold, injection test every 10min pain once. The measured pain threshold compared with the basal pain threshold, expressed as a percentage change.