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为拓宽小麦茎腐病(又称茎基腐病)抗源种类,筛选抗茎腐病小麦新种质,对43份转TaPIMP1、AtNPR1和Gastrodianin基因小麦纯合株系,进行目的基因表达分析,以及茎腐病、纹枯病和赤霉病抗性鉴定。结果表明,转基因株系的目的基因均能正常表达;转基因株系间茎腐病抗性差异明显,24份转基因株系茎腐病抗性,比受体对照扬麦12显著提高;转基因株系茎腐病抗性与纹枯病抗性相关性显著,与赤霉病相关性不显著。结合农艺性状鉴定,筛选出5份抗茎腐病转基因株系,其中2份兼抗纹枯病和赤霉病,1份兼抗纹枯病,可作为长江中下游麦区茎腐病备用抗源。
In order to broaden the resistant varieties of wheat stalk rot (also known as stem rot), new wheat germplasm with resistance to stalk rot was screened and 43 wheat homozygous transgenic lines of TaPIMP1, AtNPR1 and Gastrodianin were screened for gene expression analysis. As well as stalk rot, sheath blight and scab resistance. The results showed that the target genes of the transgenic lines could all be expressed normally. The resistance to stalk rot of the transgenic lines was significantly different. The stalk rot resistance of 24 transgenic lines was significantly higher than that of the recipient control Yangmai 12. The transgenic lines Stalk rot resistance was significantly correlated with sheath blight resistance, but not with scab. Combining with the identification of agronomic traits, 5 transgenic lines with resistance to stalk rot were screened, of which 2 were resistant to Sheath Blight and Scab, 1 were also resistant to Sheath Blight and could be used as the stalk rot reserve in the middle and lower reaches of the Yangtze River source.