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以紫皮大蒜品种‘G075’为试材,研究了鳞茎发育过程中鳞茎外皮花青苷的积累规律及与其生物合成相关的苯丙氨酸解氨酶(PAL)、查儿酮异构酶(CHI)和二氢黄酮醇还原酶(DFR)活性的关系,分析了不同设施栽培方式、基质pH值、基质氮素水平和磷素水平对花青苷生物合成的影响。研究结果表明:随着大蒜鳞茎的发育,鳞茎外皮花青苷含量逐步提高,CHI活性与鳞茎外皮花青苷积累关系密切,其活性变化与花青苷积累趋势吻合;露地栽培温度相对较低的紫皮大蒜花青苷积累高于保护地温度相对较高的栽培大蒜,基质pH 6.5和1/2氮素水平(7.5 mmol·L-1)时对花青苷合成有利,花青苷积累随磷素水平提高呈现先升后降的变化趋势。
The experiment was conducted to study the accumulation of anthocyanin in bulblets and the relationship between phenylalanine ammonia lyase (PAL) and chalcone isomerase CHI) and dihydroflavonol reductase (DFR) activity. The effects of different cultivation methods, substrate pH, substrate nitrogen and phosphorus levels on anthocyanin biosynthesis were analyzed. The results showed that with the development of garlic bulb, the content of anthocyanin in the bulb outer sheath gradually increased, and the activity of CHI was closely related to the accumulation of anthocyanin in bulb outer sheath. The change of its activity was consistent with the trend of accumulation of anthocyanin. The temperature of field cultivation was relatively low Purple garlic anthocyanin accumulation higher than the protection of the cultivation of relatively high temperature garlic matrix pH 6.5 and 1/2 nitrogen level (7.5 mmol · L-1) on anthocyanin synthesis, anthocyanin accumulation with The level of phosphorus increased first and then decreased.