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目的 探讨肿瘤坏死因子受体 (TNFR)基因转移对TNF杀伤膀胱肿瘤细胞作用的影响。 方法 建立TNFR逆转录病毒表达载体 ,通过转染包装细胞PA317产生完整病毒颗粒 ;膀胱癌BIU 87细胞经病毒感染后 ,检测细胞表面TNFR数量及TNF杀伤作用变化。 结果 TNFR基因转移前膀胱癌BIU 87细胞表面与TNF结合的位点数量平均为 912个 /细胞 ,经病毒感染后 ,细胞表面与TNF结合的位点数量平均为 2 872个 /细胞 (P <0 .0 5 ) ;TNF对经病毒感染的BIU 87细胞的杀伤作用较未经病毒感染者明显增强 (P <0 .0 5 )。 结论 以逆转录病毒为载体的基因转移方法可以提高肿瘤细胞表面TNFR的表达量 ,从而增强TNF对肿瘤细胞的杀伤作用。
Objective To investigate the effect of tumor necrosis factor receptor (TNFR) gene transfer on the cytotoxicity of TNF against bladder tumor cells. Methods TNFR retroviral vector was constructed and transfected into packaging cells PA317 to produce complete virus particles. After infected with bladder cancer BIU 87 cells, the number of TNFR and the cytotoxicity of TNF were measured. Results The average number of TNF-binding sites on the surface of bladder cancer BIU 87 cells prior to TNFR gene transfer was 912 cells / cell. After virus infection, the average number of sites on the cell surface binding to TNF was 2 872 cells / cell (P <0 .0 5). The cytotoxicity of TNF to virus-infected BIU-87 cells was significantly higher than that without virus infection (P <0.05). Conclusion Retrovirus-mediated gene transfer can increase the expression of TNFR on the surface of tumor cells and enhance the killing effect of TNF on tumor cells.