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目的 :探讨药用大黄煎剂的肠粘膜屏障保护作用机制。方法 :制备小鼠肠系膜上动脉缺血 再灌注损伤的实验模型 ,术后 6 ,12和 2 4h处死动物并留取标本 ,检测血浆总抗氧化能力、内毒素、小肠组织相对含水量和肠粘膜上皮细胞 (EC)、固有层淋巴细胞 (LPL)及Peyer’s结节淋巴细胞 (PPL)凋亡情况。结果 :药用大黄煎剂可以显著提高小鼠的血浆总抗氧化能力 (P <0 0 1) ,降低血浆内毒素水平和小肠组织含水量 (P <0 0 5 ) ,延迟并下调LPL和PPL凋亡。结论 :致伤后早期应用药用大黄煎剂对肠道粘膜屏障具有保护作用 ,其机制可能与其延迟并下调肠粘膜EC ,LPL及PPL凋亡有关
Objective: To explore the protective mechanism of intestinal mucosal barrier in medicinal Rhubarb decoction. METHODS: An experimental model of mouse superior mesenteric artery ischemia-reperfusion injury was prepared. Animals were sacrificed at 6, 12, and 24 hours after surgery and samples were taken. Plasma total antioxidant capacity, endotoxin, relative water content of intestinal tissue, and intestinal mucosa were measured. Epithelial cells (EC), lamina propria lymphocytes (LPL), and Peyer’s nodular lymphocytes (PPL) apoptosis. Results: Medicinal rhubarb decoction can significantly increase the plasma total antioxidant capacity (P <0 01), reduce plasma endotoxin levels and intestinal tissue water content (P <0 05), delay and down-regulate LPL and PPL Apoptosis. Conclusion : Early application of medicinal Rhubarb decoction has a protective effect on intestinal mucosal barrier, which may be related to its delay and down-regulation of apoptosis of intestinal mucosal EC, LPL and PPL.