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目的探讨Numb与P53蛋白在上皮性卵巢癌中的表达、相关性及临床意义。方法采用免疫组化PV二步法联合检测Numb与P53蛋白在20例正常卵巢组织、29例良性卵巢肿瘤组织、35例交界性卵巢肿瘤组织及87例上皮性卵巢癌组织中的表达,分析它们的临床意义。结果 Numb蛋白在正常卵巢组织、良性卵巢肿瘤组织、交界性卵巢肿瘤组织及上皮性卵巢癌组织中的阳性表达率分别为5%、13.8%、51.4%、70.1%,呈逐渐升高的趋势(P<0.05);不同卵巢癌分期及有无淋巴结转移的上皮性卵巢癌患者Numb蛋白表达率差异有统计学意义(P<0.05),而不同年龄、病理类型、分化程度的患者间差异无统计学意义(P>0.05)。P53蛋白在正常卵巢组织、良性卵巢肿瘤组织、交界性卵巢肿瘤组织及上皮性卵巢癌组织中的阳性表达率分别为0%、3.4%、28.6%、63.2%,呈逐渐升高的趋势(P<0.05);不同病理类型、分化程度、卵巢癌分期及有无淋巴结转移的上皮性卵巢癌患者P53蛋白表达率差异有统计学意义(P<0.05),而不同年龄间表达率差异无统计学意义(P>0.05)。Numb与P53蛋白在交界性卵巢肿瘤组织和上皮性卵巢癌组织中的表达均呈正相关(r=0.488,P<0.05;r=0.231,P<0.05)。结论通过联合检测Numb与P53蛋白可能有助于上皮性卵巢癌的早期诊断及预后判断,两种蛋白可能共同参与上皮性卵巢癌的发生发展过程。
Objective To investigate the expression, correlation and clinical significance of Numb and P53 protein in epithelial ovarian cancer. Methods The expression of Numb and P53 protein in 20 cases of normal ovarian tissue, 29 cases of benign ovarian tumor, 35 cases of borderline ovarian tumor and 87 cases of epithelial ovarian cancer were examined by immunohistochemical PV two-step method. The clinical significance. Results The positive expression rates of Numb protein in normal ovarian tissue, benign ovarian tumor, borderline ovarian tumor and epithelial ovarian cancer were 5%, 13.8%, 51.4% and 70.1% P <0.05). Numb protein expression in different stages of ovarian cancer and lymph node metastasis in patients with epithelial ovarian cancer was significantly different (P <0.05), but no statistical difference was found between different age, pathological types and differentiation Significance (P> 0.05). The positive rates of P53 protein in normal ovarian tissue, benign ovarian tumor, borderline ovarian tumor and epithelial ovarian cancer were 0%, 3.4%, 28.6% and 63.2% <0.05). There was significant difference in the expression of P53 protein in different pathological types, differentiation degree, stage of ovarian cancer and lymph node metastasis (P <0.05), but there was no significant difference in the expression rate of P53 between different age groups Significance (P> 0.05). There was a positive correlation between Numb and P53 protein in borderline ovarian cancer and epithelial ovarian cancer (r = 0.488, P <0.05; r = 0.231, P <0.05). Conclusion The combination of Numb and P53 protein may contribute to the early diagnosis and prognosis of epithelial ovarian cancer. Both proteins may participate in the development and progression of epithelial ovarian cancer.