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80只二级BALB/c小鼠随机分为二大组:实验组动物接种猫胃螺杆菌(HF)建立HF动物模型,感染HF16周后给予10％NaCl灌喂2次/周、25μg/ml N-甲基-N′-硝基-N-亚硝基胍(MNNG)自由饮用共12周,利用抗Brdu免疫组化、流式细胞计等技术比较各组的细胞动力学指标,结果实验组所有动物均感染HF,胃粘膜表现为慢性活动性胃炎;而对照组仅有极少数炎性细胞浸润。实验组各小组的标记指数LI、S期细胞比例S％、增殖指数PI均比对照组高(P<0.01)。
Eighty secondary BALB/c mice were randomly divided into two groups: Animals in the experimental group were inoculated with Helicobacter felis (HF) to establish an animal model of HF. After 16 weeks of infection with HF, 10% NaCl was given twice per week, and 25 μg/ml. N-methyl-N’-nitro-N-nitrosoguanidine (MNNG) was allowed to freely drink for 12 weeks. The cell kinetic parameters of each group were compared using anti-Brdu immunohistochemistry and flow cytometry techniques. All animals in the group were infected with HF, and the gastric mucosa showed chronic active gastritis; while the control group had only a few inflammatory cell infiltrations. The labeling index LI, S phase cell ratio S%, and proliferation index PI of each group in the experimental group were higher than those in the control group (P<0.01).