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目的:探讨麻杏石甘汤以及汤剂中聚集物体外对A型流感病毒活性的影响。方法:运用狗肾上皮(MDCK)细胞培养、MTT比色法检测7 200r/min离心上清经0.45μm膜过滤前后麻杏石甘汤蒸馏水煎剂样品体外对A型流感病毒活性变化;运用动态光散射粒度分析仪、场发射扫描电子显微镜分析过滤前后麻杏石甘汤以及聚集物的粒径及形貌特征。结果:麻杏石甘汤0.45μm膜过滤前后,对流感病毒感染MDCK细胞治疗指数(TI)分别为:3.65,0.27,膜过滤明显降低了滤过液的抗病毒活性;动态光散射测定结果表明,膜过滤去除了大量粒径较大的聚集物,明显缩小了体系的粒径分布范围,过滤后,体系平均粒径下降约70.00nm;电镜观察表明,粒径>500.00nm的聚集物多截留于膜上。结论:麻杏石甘汤体外抗A型流感病毒活性与其汤剂中聚集物具有相关性。
Objective: To investigate the effect of Maxingshigan Decoction and aggregates in decoction on the activity of influenza A virus in vitro. Methods: MTT assay was used to detect the changes of influenza A virus activity of Maxingshigan Decoction before and after filtering by 0.45μm membrane in 7200r / min centrifugation supernatant. Light scattering particle size analyzer, field emission scanning electron microscopy analysis before and after the Maxing Shigan Decoction and aggregate size and morphology characteristics. Results: Before and after treatment with Maxing Shigan Decoction, the therapeutic index (TI) of MDCK cells infected by influenza virus were 3.65 and 0.27, respectively. Membrane filtration significantly reduced the antiviral activity of the filtrate. The results of dynamic light scattering , Membrane filtration to remove a large number of larger aggregates, significantly reducing the size distribution of the system, after filtration, the average particle size decreased about 70.00nm; electron microscopy showed that the particle size> 500.00nm aggregate interception On the membrane. Conclusion: Maxingshigan Decoction in vitro anti-influenza A virus activity and its decoction of aggregates have relevance.