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目的:观察调衡方对Lewis肺癌细胞增殖凋亡及JAK-STAT通路的影响。方法:实验动物分为4组,灌服给药,制备调衡方小、中、大剂量含药血清,正常血清。又将细胞分为5组,即正常血清组(不含药血清),小、中、大剂量含药血清组,中剂量含药血清+AG490(JAK-STAT信号通路特异性抑制剂)干预组,分别孵育Lewis肺癌细胞株24 h后,MTT法检测对Lewis肺癌细胞增殖的影响,吖啶橙染色观察Lewis肺癌细胞凋亡状况,RT-PCR法检测其对Lewis肺癌细胞Stat3、Stat5 mRNA的影响。结果:Lewis肺癌细胞经调衡方中、大剂量含药血清作用后,与对照组比较,细胞内Stat3含量降低至0.622±0.004、0.62±0.007(P<0.01),Stat5表达分别降低至0.700±0.034、0.649±0.033(P<0.01)。结论:调衡方含药血清能显著抑制Lewis肺癌细胞增殖,促进其凋亡,其机制可能与下调stat3、stat5的基因表达,抑制细胞信号转导通路有关。
OBJECTIVE: To observe the effect of Regulating Formula on proliferation, apoptosis and JAK-STAT pathway of Lewis lung cancer cells. Methods: The experimental animals were divided into 4 groups and administered by gavage to prepare small, medium and large doses of drug-containing serum and normal serum. The cells were divided into 5 groups: normal sera (without drug serum), small, medium, and large doses of drug-containing serum, and medium-dose drug-containing serum + AG490 (specific inhibitor of JAK-STAT signaling pathway) intervention group. After incubation with Lewis lung cancer cells for 24 h, the proliferation of Lewis lung cancer cells was detected by MTT assay. Apoptosis of Lewis lung cancer cells was observed by acridine orange staining. The effect of Lewis acid on Stat3 and Stat5 mRNA expression in Lewis lung cancer cells was detected by RT-PCR. . Results: Compared with the control group, the Stat3 content of Lewis lung cancer cells decreased to 0.622±0.004, 0.62±0.007 (P<0.01), and the expression of Stat5 decreased to 0.700±, respectively. 0.034, 0.649±0.033 (P<0.01). Conclusion: The serum containing the balance can significantly inhibit the proliferation of Lewis lung cancer cells and promote its apoptosis. The mechanism may be related to the down-regulation of stat3, stat5 gene expression and inhibition of cell signal transduction pathways.