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采用体外培养法探讨提取的紫苏挥发油对人肺癌LTEP-a-2细胞的生长抑制作用。设计了3组紫苏挥发油药物组,各药物组质量浓度均为1、3、5、8、10、15、20、25、30 mg/mL,各组在分别培养24、48、72 h后,利用MTT法测试紫苏挥发油对人肺癌细胞生长的抑制作用。结果表明,紫苏挥发油在体外实验中具有抑制人肺癌LTEP-a-2细胞生长的作用,且呈剂量和时间依赖性,(20~30)mg/mL时可以作为其抑制LTEP-a-2细胞生长的最佳参考浓度。
In vitro culture was used to investigate the inhibitory effects of the extracted volatile oil of perilla on the growth of human lung cancer LTEP-a-2 cells. Three groups of perilla essential oil drug groups were designed. The mass concentration of each drug group was 1, 3, 5, 8, 10, 15, 20, 25 and 30 mg / mL. , The inhibition of the growth of human lung cancer cells by volatile oil of perilla was tested by MTT method. The results showed that the volatile oil of Perilla frutescens L. could inhibit the growth of LTEP-a-2 cells in vitro in a dose-and time-dependent manner and could be used as an inhibitor of LTEP-a-2 in (20-30) mg / mL The best reference concentration for cell growth.