国外早在60年代羊水胎儿细胞培养获得成功,并作了核型分析,为子宫内诊断预防遗传性疾病,提供了新的途径。70年代中国科学院遗传研究所等也开展了此项研究。我所自1980年6月开始摸索羊水细胞培养技术。到同年11月已初步培养成功,并显出清晰的核型。1981年6月我所开展羊水胎儿细胞培养,进行产前诊断,至今已做了20例。现将其培养技术介绍如下。 Abroad as early as 60 years of amniotic fluid fetal cell culture success, and made karyotype analysis, for the prevention of intrauterine diseases genetic disease, provides a new way. The Institute of Genetics of the Chinese Academy of Sciences also conducted the study in the 1970s. I am from June 1980 began to explore amniotic fluid cell culture technology. By the same year in November has initially cultured successfully, and showed a clear karyotype. June 1981 I carried out amniotic fluid fetal cell culture, prenatal diagnosis, has been done in 20 cases. Now its training techniques are as follows.