论文部分内容阅读
目的观察保罗样激酶1基因(plk1)沉默对胶质瘤细胞株-H4体外生长的抑制作用,探讨plk1基因作为胶质瘤治疗靶点的可行性。方法化学合成小片断干扰RNA(siRNA)抑制plk1基因的表达,Western blot检测plk1蛋白质的表达变化,流式细胞仪检测H4细胞周期分布及凋亡程度的变化,体外侵袭实验检测H4细胞侵袭能力的变化,MTT法检测H4细胞增殖速度的变化。结果经siRNA作用48h后,plk1蛋白质水平明显降低;较多的H4细胞聚集于G2/M期附近(P<0.05);细胞凋亡明显上升(P<0.05);细胞体外侵袭能力下降(P<0.05);增殖速度明显缓于对照组(P< 0.05)。结论靶向plk1的siRNA可在体外抑制胶质瘤细胞H4的侵袭与增殖,plk1有可能成为新的潜在胶质瘤治疗靶点。
Objective To observe the inhibitory effect of silencing plk1 gene on glioma cell line -H4 in vitro and to explore the feasibility of plk1 gene as a target for glioma therapy. Methods Small interfering RNAs (siRNAs) were chemically synthesized to inhibit the expression of plk1 protein. The protein expression of plk1 was detected by Western blot. The cell cycle distribution and apoptosis of H4 cells were detected by flow cytometry. The invasiveness of H4 cells was evaluated by in vitro invasion assay Changes, MTT assay H4 cell proliferation rate changes. Results After treated with siRNA for 48 hours, the level of plk1 protein decreased significantly. More H4 cells gathered in the vicinity of G2 / M phase (P <0.05), apoptosis increased significantly (P <0.05), and the invasive ability of cells decreased in vitro (P < 0.05). The proliferation rate was significantly slower than the control group (P <0.05). Conclusion siRNA targeting plk1 can inhibit the invasion and proliferation of H4 in glioma cells in vitro, and plk1 may be a potential therapeutic target for potential glioma.