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目的探讨雌激素受体(ER)亚型α和β mRNA及蛋白的异常表达与子宫内膜癌的发生发展的关系。方法应用半定量逆转录聚合酶链反应(RT-PCR)和免疫组织化学链霉菌抗生物素蛋白-过氧化酶连接法(SP法),检测18例正常子宫内膜组织、29例异常增生组织和50例子宫内膜癌组织中ERα、ERβ mRNA及蛋白的表达。结果①ERα mR-NA在内膜癌组织中的阳性表达率和阳性表达水平明显低于异常增生内膜组织及正常内膜组织,三者比较有显著性差异(P<0.01),ERα蛋白在这三组间的表达率变化相同(P<0.05)。②ERβ mRNA在内膜癌组织中的阳性表达率和阳性表达水平明显低于增生性内膜组织及正常内膜组织,三者比较有显著性差异(P<0.01),ERβ蛋白在这三组间的表达率变化相同(P<0.01)。③ERα和ERβ mRNA平均表达水平比值(ERα/ERβ)在内膜癌组低于异常增生子宫内膜组织和正常子宫内膜组(P<0.05)。结论ERα和ERβ表达的下调可能与子宫内膜癌的发生有关,在这个过程中ERα的下调起的作用大于ERβ。
Objective To investigate the relationship between abnormal expression of α and β mRNA and protein of estrogen receptor (ER) and the occurrence and development of endometrial carcinoma. Methods 18 cases of normal endometrium and 29 cases of abnormal tissue were detected by semi-quantitative RT-PCR and immunohistochemical streptavidin-peroxidase (SP method) And 50 cases of endometrial cancer ERα, ERβ mRNA and protein expression. Results ① The positive expression rate and positive expression level of ERα mR-NA in endometrial carcinoma were significantly lower than those in abnormally proliferated endometrium and normal endometrium (P <0.01) The expression of the three groups changed the same (P <0.05). ② The positive rate and positive expression level of ERβ mRNA in endometrial carcinoma tissues were significantly lower than those in proliferative endometrium and normal endometrial tissues (P <0.01). ERβprotein expression in these three groups (P <0.01). ③ The ratio of ERα / ERβ mRNA expression in endometrial carcinoma was lower than that in dysplastic endometrium and normal endometrium (P <0.05). Conclusion The down-regulation of ERα and ERβ may be related to the occurrence of endometrial carcinoma. ERα is down-regulated more than ERβ in this process.