建立了新疆紫草萘醌类总成分的毛细管电泳分离方法,考察了检测波长、添加剂类型、进样量和分离电压对分离检测的影响。实验条件为检测波长200nm,运行缓冲液为含20%乙醇的硼酸盐缓冲液(0.1 mol/L,pH 8.0),进样量3.45kPa×5s,分离电压20kV。方法可在20 min内检测到5种活性成分,重现性良好。 A capillary electrophoresis separation method was established for the determination of total naphthoquinones in Lithospermum officinale. The influences of detection wavelength, additive type, injection volume and separation voltage on the separation and detection were investigated. The experimental conditions were as follows: the detection wavelength was 200 nm, the running buffer was 20% ethanol borate buffer (0.1 mol / L, pH 8.0), the injection volume was 3.45 kPa × 5 s and the separation voltage was 20 kV. The method can detect five kinds of active ingredients in 20 min, with good reproducibility.