目的探讨酒后小白鼠生殖系统组织标本的制作方法。方法取20只饮酒3个月后的雄性小白鼠的睾丸、附睾、阴茎组织各切2块分成2组,第1组经10%甲醛固定,第2组经自配固定液固定(苦味酸饱和水溶液50mL,甲醇40mL,冰醋酸10mL)、包埋、切片、HE染色。结果自配固定液组组织平整、入水不散、易连续切片,染色鲜艳,胞核清晰。结论自配液组织制作方法优于甲醛固定制作方法。 Objective To explore the method of making genital tissue samples of drinking mice. METHODS: Twenty male and female mice were divided into two groups, testis, epididymis and penile tissue. The first group was fixed with 10% formalin and the second group was fixed with self-fixative solution (picric acid saturation Aqueous solution 50mL, methanol 40mL, glacial acetic acid 10mL), embedded, sectioned, HE staining. Results Self-fixed fluid group formation, water is not scattered, easy to slice, brightly colored, clear nucleus. Conclusion Self-dosing method is better than formaldehyde fixation method.