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目的研究ICRF154和加热对腺样囊性癌ACC－2细胞放射增敏的作用。方法以腺样囊性癌ACC－2细胞作为实验模型，用新抗癌药ICRF154在乏氧条件下研究该药的放射增敏性以及加热对放射的增敏效应。结果在0．2mmol／L浓度和2个小时作用时间下，ICRF154对ACC－2细胞有放射增敏作用，增敏比为1．22、放射后加热43℃，30分钟对ACC－2细胞有显著的放射增敏作用，增敏比为1．51。用上述浓度和作用时间ICRF154，作用于ACC－2细胞后进行照射，照华后即行加热43℃，30分钟，增敏比为1．67，表明ICRF154和加热的放射增敏作用有加成效应。结论ICRF154的放射增敏机制可能与其抑制拓扑异构酶Ⅱ，从而抑制DNA损伤的修复有关。
Objective To investigate the radiosensitization effect of ICRF154 and heat on adenoid cystic carcinoma ACC-2 cells. Methods The adenoid cystic carcinoma ACC-2 cells were used as the experimental model to study the radiosensitization of the drug and the sensitizing effect of heating to radiation with the new anticancer drug ICRF154 under hypoxic conditions. Results ICRF154 had a radiosensitizing effect on ACC-2 cells at a concentration of 0.2mmol / L and an action time of 2 hours, with a sensitization ratio of 1.22. After irradiation, the cells were heated at 43 ℃ for 30 minutes Significant radiosensitization, sensitization ratio of 1.51. ICRF154 was used to irradiate ACC-2 cells with the above concentration and action time. After irradiation, the cells were heated at 43 ° C for 30 minutes and the sensitization ratio was 1.67, indicating that ICRF154 and heating radiosensitization have an additive effect . Conclusion The radiosensitization mechanism of ICRF154 may be related to the inhibition of topoisomerase Ⅱ and thus the repair of DNA damage.