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为探讨小细胞肺癌（ＳＣＬＣ）发生的分子机制，应用免疫组化和聚合酶链反应—单链构象多态性分析的方法，对１４例ＳＣＬＣ组织进行ｐ５３基因突变研究。结果显示，有９例出现ｐ５３蛋白阳性，阳性率为６４．３％（９／１４）。ｐ５３基因第５，６，７，８外显子突变率分别为２１．４％（３／１４），１４．３％（２／１４），１４．３％（２／１４），７．１％（１／１４），总突变率为５７．１％（８／１４）。结果表明，ＳＣＬＣ组织中存在较高的ｐ５３基因突变率，故ｐ５３基因可能是人类肺癌发生的关键基因。
In order to investigate the molecular mechanism of SCLC, immunohistochemistry and polymerase chain reaction-single strand conformation polymorphism analysis were used to investigate mutations of p53 gene in 14 SCLC tissues. The results showed that there were 9 cases of p53 protein positive, the positive rate was 64.3% (9/14). The mutation rates of exon 5, 6, 7, and 8 in p53 gene were 21.4% (3/14), 14.3% (2/14), and 14.3% (2/14), respectively. % (1/14), the total mutation rate was 57.1% (8/14). The results show that there is a high mutation rate of p53 gene in SCLC tissues, so p53 gene may be a key gene in the development of human lung cancer.