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为探讨小细胞肺癌(SCLC)发生的分子机制,应用免疫组化和聚合酶链反应—单链构象多态性分析的方法,对14例SCLC组织进行p53基因突变研究。结果显示,有9例出现p53蛋白阳性,阳性率为64.3%(9/14)。p53基因第5,6,7,8外显子突变率分别为21.4%(3/14),14.3%(2/14),14.3%(2/14),7.1%(1/14),总突变率为57.1%(8/14)。结果表明,SCLC组织中存在较高的p53基因突变率,故p53基因可能是人类肺癌发生的关键基因。
In order to investigate the molecular mechanism of SCLC, immunohistochemistry and polymerase chain reaction-single strand conformation polymorphism analysis were used to investigate mutations of p53 gene in 14 SCLC tissues. The results showed that there were 9 cases of p53 protein positive, the positive rate was 64.3% (9/14). The mutation rates of exon 5, 6, 7, and 8 in p53 gene were 21.4% (3/14), 14.3% (2/14), and 14.3% (2/14), respectively. % (1/14), the total mutation rate was 57.1% (8/14). The results show that there is a high mutation rate of p53 gene in SCLC tissues, so p53 gene may be a key gene in the development of human lung cancer.