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应用质粒载体ｐＵＣ１８构建赖型钩端螺旋体０１７株的基因库。筛选出重组质粒ｐＤＪ６和ｐＤＪ８，插入片段分别是１．９ｋｂ和２．２ｋｂ。地高辛随机引物标记１．９ｋｂ片段制备探针，对５个血清型６株钩体ＤＮＡ进行杂交。结果显示，重组探针与致病钩体ＤＮＡ出现明显杂交信号，与非致病钩体，人白细胞ＤＮＡ及大肠杆菌ＪＭ１０３株ＤＮＡ杂交信号不明显。此特异性重组探针可作为鉴定、识别致病性钩体和进行钩体属、种分类的一种手段。
Gene library of 017 strain of Leptospira interrogans was constructed using plasmid vector pUC18. The recombinant plasmids pDJ6 and pDJ8 were screened, and the inserted fragments were 1.9kb and 2.2kb, respectively. Digoxigenin primers were used to label the 1.9kb fragment for hybridization of six serogroups of leptospira DNA. The results showed that there was obvious hybridization signal between the recombinant probe and the pathogenic Leptospira DNA, and the signal of hybridization with the non-pathogenic leptospira, human leukocyte DNA and E. coli JM103 DNA was not obvious. This specific recombinant probe can be used as identification, identification of pathogenic leptospira and leptospirosis, species classification as a means.