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将离体细胞在实验室中进行长期培养,建立体外实验模型的组织培养技术,已广泛应用于基础医学科学的研究。培养细胞的保种则是细胞株/系建立后的一个重要问题。组织培养中常用传代培养法和低温冻存法。比较而言,前者不仅耗费了大量人力、物力、而且难以避免微生物污染等意外,甚至发生细胞生物学特征的改变。为此,我们对几个细胞系进行液氮低温(—196℃)冻存。现将液氮中成功冻存4年的高转移性人肺腺癌 Anip—973细胞系的冻存及复苏培养情况简介如下。
The long-term culture of ex vivo cells in the laboratory, the establishment of in vitro experimental model of tissue culture technology, has been widely used in basic medical science research. Conservation of cultured cells is an important issue after the establishment of cell lines/lines. Tissue culture is commonly used in subculture and cryopreservation. In comparison, the former not only consumes a lot of manpower and material resources, but it is difficult to avoid accidents such as microbial contamination, and even changes in the characteristics of cell biology. For this purpose, we cryopreserved several cell lines at low temperature (-196°C) in liquid nitrogen. The cryopreservation and resuscitation culture of highly metastatic human lung adenocarcinoma cell line Anip-973, which has been successfully frozen in liquid nitrogen for 4 years, is described below.