介绍一种旋转表面涂布活菌计数方法,具有螺旋平板法及表面涂布法的优点。经此法处理标本,菌落均匀分布于平板上。所得结果与传统的倾注平板法比较,有良好的相关性。双份平板计数之间差异无显著性。本法菌落形成较早,15～18h即可计数,从而缩短了报告时间,且操作简便,无需复杂设备。 A rotary surface coating method for counting viable bacteria is introduced, which has the advantages of spiral plate method and surface coating method. After this method to deal with specimens, colonies evenly distributed on the plate. The results obtained compared with the traditional pour plate method, there is a good correlation. There was no significant difference between double plate count. This method of colony formation earlier, 15 ~ 18h can count, thus shortening the reporting time, and easy to operate, without complex equipment.