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CA3区的锥体细胞是海马内部联系的关键环节,且对缺血、衰老等伤害因素最为易感。研究CA3神经元的年龄变化,对于探索海马的结构和功能的衰老机制有重要意义。雄性Wistar大鼠40只,分为幼年(1月)、青年(3月)、成年(17月)、和老年(30月)4组。4％福尔马林PBS灌注(pH 7.2),取脑后石蜡切片12 μm。取一套切片行Brachet法显示RNA,每例选10个有核仁的CA_3锥体细胞(每组100个),经MPV_3显微分光光度计测定RNA含量(双波法)。同时测量核仁直径,按球体公式计算体积,并计算250 μm段的锥体细胞数。另一套切片脱蜡后石蜡油封片,荧光显微镜观察衰老色素(LP),每组选60个CA3锥体细胞荧光光度法测定LP含量。实验结果:1.海马神经细胞胞浆和核的RNA染
Pyramidal cells in the CA3 region are the key links in the hippocampal internal connection and are most susceptible to injury factors such as ischemia and aging. Studying age changes of CA3 neurons is of great significance for exploring the mechanism of aging of the structure and function of the hippocampus. Forty male Wistar rats were divided into 4 groups: infancy (January), youth (March), adulthood (17 months), and old age (30 months). 4% formalin-PBS (pH 7.2), paraffin section 12 μm. RNA was extracted from a set of sections by Brachet method. Ten nucleated CA_3 pyramidal cells (100 in each group) were selected for each study. RNA content (double wave method) was determined by MPV_3 spectrophotometer. The diameter of nucleoli was measured at the same time, the volume was calculated according to the sphere formula, and the number of pyramidal cells in 250 μm segments was calculated. Another set of deparaffinization paraffin oil seal pieces, observed under the fluorescent microscope aging pigment (LP), each group of 60 selected CA3 pyramidal cell fluorescence spectrophotometry LP content. Experimental results: 1. RNA staining of the cytoplasm and nucleus of hippocampal neurons