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原发性痛经被认为是与性激素紊乱伴随月经前的孕酮减少有关。下丘脑的单胺和其代谢产物可能参与了这个病理过程。用微透析结合HPLC-ECD快速鉴定正常和原发性痛经模型大鼠下丘脑中的5-HIAA。微透析样品中5-HIAA的检测限和定量限分别是1.5ng/mL和5.0ng/mL。校准曲线在10-250ng/mL的浓度范围内为线性。痛经模型大鼠的5-HIAA的含量和正常对照组相比有显著的增加,表明原发性痛经导致5-HIAA的增加。经过香附四物汤的治疗,5-HIAA的含量降低,显示香附四物汤通过降低下丘脑的5-羟色胺的代谢率从而减轻原发性痛经的疼痛综合症。
Primary dysmenorrhea is thought to be associated with a decrease in progesterone associated with pre-menstrual disturbances of sex hormones. The hypothalamus monoamine and its metabolites may be involved in this pathological process. Rapid identification of 5-HIAA in the hypothalamus of rats with normal and primary dysmenorrhea using microdialysis combined with HPLC-ECD. The limits of detection and quantitation limits for 5-HIAA in microdialysis samples were 1.5 ng / mL and 5.0 ng / mL, respectively. The calibration curve is linear over the concentration range of 10-250 ng / mL. The content of 5-HIAA in rats with dysmenorrhoea significantly increased compared with the normal control group, indicating that primary dysmenorrhea leads to the increase of 5-HIAA. After the treatment of Xiangfu Siwu Decoction, the content of 5-HIAA decreased, indicating that Xiangfu Siwu Decoction can reduce the pain syndrome of primary dysmenorrhea by lowering the metabolic rate of hypothalamic serotonin.