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以一株粉红粘帚霉(Clonostachys rosea)CAYU-3菌株为试材,采用平板培养的评价方法,研究了该菌株在6种农产品(大豆、大米、玉米、小麦、马铃薯、花生)培养基(去渣或留渣)以及PDA培养基上的菌落生长和产孢情况。结果表明:该菌株在不同农产品培养基上的菌丝生长和菌落形态存在较大的差异。无论培养基是否留渣,在大豆、花生培养基上,该菌株的菌丝生长紧密,菌丝量多,而在玉米、大米、小麦培养基中菌丝疏松,菌丝量少,整体生长状况较差。在产孢方面,该菌株在不同的农产品培养基上产孢量存在显著差异。去渣处理对该菌株产孢量有显著影响。在去渣培养基中,花生培养基的产孢量最高(1.79×107个孢子·cm-2);在留渣培养基中,大豆培养基的产孢量最高(2.87×107个孢子·cm-2)。由此看来,大豆和花生培养基较适合该菌株的产孢。试验结果还表明,大米培养基对该菌株的产孢不是很有利,无论去渣与否,大米培养基的产孢量均较低(0.24×107~0.32×107个孢子·cm-2)。
A strain of Corynebacterium glutamicum CAYU-3 was used as material to evaluate the effects of this strain on six kinds of agricultural products (soybean, rice, corn, wheat, potato and peanut) To slag or residue) and colony growth and sporulation on PDA medium. The results showed that there was a big difference in the mycelial growth and colony morphology of the strain on different agricultural products. Regardless of whether the medium left residue, in the soybean, peanut medium, the strain of mycelial growth close, the amount of mycelium, and in maize, rice, wheat mycelium loose, less mycelium, the overall growth status Poor. In sporulation, the strain on different agricultural products spores on the amount of significant differences exist. Deslagging treatment had a significant effect on sporulation of this strain. The highest amount of sporulation in peanut medium (1.79 × 107 spores · cm-2) was found in the de-residue medium, while that in soybean medium was the highest (2.87 × 107 spores · cm- 2). From this, soybean and peanut medium is more suitable for sporulation of this strain. The results also showed that rice medium was not favorable for the sporulation of this strain. The amount of sporulation in rice medium was lower (0.24 × 107 ~ 0.32 × 107 spores · cm-2), regardless of the residue removal.