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目的：建立硼砂酚醛器械消毒液中甲醛的含量测定法和确定器械液的存贮期限。方法：采用衍生紫外法测定器械液中甲醛的含量，并采用恒温法预测器械液的有效期，同时利用金黄色葡萄球菌和绿脓杆菌观察不同贮放期间的抑菌效果。结果：衍生紫外法在４１３．０ｎｍ 处测定该制剂中甲醛的含量，回收率１００．１％ ，ＲＳＤ ０．３７％ ，其它组份无干扰；苯酚有效期４１ ｄ，甲醛有效期６１ ｄ；光线、氧和微量金属离子Ｆｅ３＋ 、Ｃｕ２＋ 使溶液颜色发生变化；１５ｄ 后器械液虽有变色，但３０ ｄ 内不影响其抑菌活性。结论：衍生紫外法测定器械液中甲醛的含量较容量法准确可靠，适合医院快检要求；金属离子、光线、氧和温度可加速器械液的变色反应，应控制原料质量与贮存条件；器械液应新鲜配制，１５ ｄ 内定期更换，贮用期最长不超过１ 个月
Objective: To establish a method for the determination of formaldehyde in disinfectant of borax phenolic device and to determine the storage period of instrument liquid. Methods: The content of formaldehyde in the instrument liquid was determined by the derivatization UV method. The thermostat method was used to predict the validity of the instrument liquid. At the same time, the antibacterial activity of Staphylococcus aureus and Pseudomonas aeruginosa were observed. Results: The content of formaldehyde in the preparation was determined by the derivatization UV method at 413.0nm. The recovery rate was 100.1%, RSD 0.37%, the other components had no interference; the phenol was effective for 41 days and the formaldehyde was for 61 days; And trace metal ions such as Fe3 + and Cu2 + changed the solution color. After 15 days, the instrument liquid changed color, but did not affect the antibacterial activity within 30 days. Conclusions: The determination of formaldehyde content by UV method is more accurate and reliable than the volumetric method, which is suitable for hospital fast inspection requirements. Metal ions, light, oxygen and temperature can accelerate the discoloration reaction of the device liquid. The quality and storage conditions of the device should be controlled. Should be freshly prepared, 15d within a regular replacement, storage period of not more than 1 month