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目的:探讨白藜芦醇对6-羟基多巴引起的细胞损伤的内在保护机制。方法:以SN4741细胞系为实验对象,分为对照组、6-羟基多巴处理组和白藜芦醇预处理、6-羟基多巴处理组组。MTT法测定细胞活性。Western blot检测细胞内DJ-1表达水平。ROS检测反映细胞的氧化应激水平和线粒体损伤情况。线粒体膜电位检测反映细胞线粒体功能。结果:白藜芦醇可以剂量依赖性方式提高6-OHDA诱导的SN4741细胞的存活率。白藜芦醇预处理显著逆转6-OHDA诱导的SN4741细胞DJ-1水平的下降,降低6-OHDA引起的氧化应激水平和线粒体损伤。结论:白藜芦醇预处理能够保护6-羟基多巴所致的SN4741细胞损伤,可能与提高DJ-1的表达,减轻细胞内的氧化应激水平,改善线粒体功能有关。
Objective: To investigate the intrinsic protective mechanism of resveratrol on 6-hydroxydopamine-induced cell injury. Methods: The SN4741 cell line was divided into control group, 6-hydroxydopamine treatment group and resveratrol pretreatment, 6-hydroxydopamine treatment group. Cell viability was measured by MTT assay. Western blot detection of intracellular expression of DJ-1. ROS detection reflects the level of cellular oxidative stress and mitochondrial damage. Mitochondrial membrane potential test reflects the mitochondrial function. Results: Resveratrol increased the survival rate of 6-OHDA-induced SN4741 cells in a dose-dependent manner. Resveratrol significantly reversed the decrease of DJ-1 level induced by 6-OHDA in SN-741 cells and decreased the level of oxidative stress and mitochondrial damage induced by 6-OHDA. CONCLUSION: Resveratrol can protect the SN4741 cells induced by 6-hydroxydopamine, which may be related to the increase of DJ-1 expression, the reduction of intracellular oxidative stress and the improvement of mitochondrial function.