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目的 :评价雷公藤内酯醇抗人肝癌SMMC 772 1细胞的作用。方法 :采用体外细胞培养和SD大鼠体内肿瘤接种两种衡量方法 ,体外细胞培养检测指标包括细胞形态观察 ,台盼蓝染色 ,DAPI染色 ,DNAladder ;SD大鼠体内接种肿瘤指标检测用游标卡尺测量肿瘤体积大小。结果 :与对照组相比 ,体外培养肿瘤细胞在用药后活细胞大幅减少 ,体内接种肿瘤细胞在用药后 ,肿瘤块体积较小且增长缓慢 ,而对照组肿瘤体积却大幅增长。结论 :雷公藤内酯醇对体外和体内培养的人肝癌SMMC 772 1细胞有明显的生长抑制作用和促凋亡作用
Objective: To evaluate the effect of triptolide on human hepatoma SMMC 772 1 cells. Methods: In vitro cell culture and in vivo tumor inoculation of SD rats were used to measure two kinds of measurement methods. Cell culture in vitro indicators including cell morphology observation, trypan blue staining, DAPI staining, DNAladder; Sprague-Dawley rats inoculated with tumor indicators measured by vernier calipers Size Results: Compared with the control group, the number of viable cells in the cultured tumor cells significantly decreased after inoculation. After inoculation of the tumor cells in vivo, the tumor volume was smaller and grew slowly, but the tumor volume of the control group increased significantly. Conclusion: Triptolide has obvious growth inhibitory and pro-apoptotic effects on human hepatoma SMMC-772 1 cells cultured in vitro and in vivo