人癌的Xenograft是将手术标本移植到裸鼠身上,并成为可传代的瘤株,实验的条件高,周期长且成功率低,一般在20～30％,最易成功的黑色素瘤也只有45％左右的成功率。有的瘤株难于消化及分散也给实验的应用带来极大的障碍。 作者用BALB/CATcL品系——本实验室自己繁殖的裸小鼠,在无菌条件下将本院外科手术标本——人结肠癌(WRJ)切成2×2×1mm~3小块,用套管针将其接种于裸鼠的腹股沟皮下,53天后长为10×9×11mm~3的 Xenograft is a human cancer transplanted into nude mice and becomes a passable tumor strain. The experimental conditions are high, the cycle is long and the success rate is low, generally 20 to 30%, and the most successful melanoma is only 45. % of success rate. Some tumor strains are difficult to digest and disperse, which also brings great obstacles to the application of the experiment. The author used the BALB/CATcL strain, a nude mouse self-bred in this laboratory, to cut the surgical specimen of this hospital, human colon cancer (WRJ) into 2 x 2 x 1 mm to 3 small pieces under aseptic conditions. The trocar inoculates the nude mouse’s groin subcutaneously, and the length is 10×9×11mm~3 after 53 days.