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为研究水稻植株中木聚糖酶抑制剂基因RIXI过量表达是否会引起其他基因的差异表达,利用转录组测序(RNA-Seq)技术结合数字基因表达谱分析对RIXI过量表达单拷贝纯合株系R7进行基因差异表达分析。水稻全基因表达谱显示,RIXI过表达引起水稻中大量基因的表达差异,包括391个上调基因,905个下调基因。GO分析将差异基因分成30个功能聚类,其中的5组聚类中含有较多的差异基因,分别为单个有机体代谢过程、生物调控、阴离子结合、小分子结合和核苷酸结合。利用KEGG数据库,通过Pathway显著性富集确定差异表达基因参与主要生化代谢途径和信号转导途径。与整个水稻基因组背景WT相比,R7的差异表达基因包含在98个KEGG通路中,包含差异基因数量最多的4个KEGG通路分别为代谢通路、次级代谢的生物合成、植物与病原菌互作和激素信号传导。农艺性状测量显示,RIXI过表达对水稻生长发育几乎没有影响。以上结果说明,木聚糖酶抑制剂基因RIXI可能在各种生物和非生物胁迫中激活复杂的信号传导,但对水稻的生长和发育没有负面影响。
In order to study whether RIXI overexpression in rice plants caused differential expression of other genes, the transcriptome sequencing (RNA-Seq) combined with digital gene expression profiling analysis of RIXI overexpression single copy homozygous lines R7 for gene differential expression analysis. The rice total gene expression profile showed that RIXI over-expression caused a large number of genes in rice, including 391 up-regulated genes and 905 down-regulated genes. GO analysis divided the differentiated genes into 30 functional clusters, among which 5 groups contained more different genes, which were single organism metabolic process, biological regulation, anion binding, small molecule binding and nucleotide binding. The KEGG database was used to identify differentially expressed genes involved in major biochemical metabolic pathways and signal transduction pathways through Pathway significant enrichment. Compared with whole genome of WT, the differentially expressed genes of R7 are contained in 98 KEGG pathways, and the four KEGG pathways containing the largest number of differential genes are metabolic pathways, secondary metabolites biosynthesis, plant-pathogen interaction and Hormonal signaling. Agronomic traits showed that RIXI overexpression had little effect on rice growth and development. The above results indicate that the xylanase inhibitor gene RIXI may activate complex signaling in various biological and abiotic stresses but has no negative impact on rice growth and development.