论文部分内容阅读
本文以抗除草剂(EPSPS)转基因大豆GTS-40-3-2为材料,在Lectin基因的扩增体系中设置了不同浓度的十二烷基硫代硫酸钠(SDS)和酚,探讨了SDS和酚这两种蛋白质变性剂在转基因成分检测中的抑制作用。结果表明,在反应体系中SDS的浓度大于或等于4.8×10-4g/mL时,能完全抑制PCR反应;酚的体积比大于或等于8.0×10-3时,体系中的DNA聚合酶全部变性,PCR反应彻底被抑制。
In this paper, the anti-herbicide (EPSPS) genetically modified soybean GTS-40-3-2 as materials, Lectin gene amplification system set different concentrations of sodium dodecylthiosulfate (SDS) and phenol, SDS And phenol two denaturant agents in the detection of genetically modified components of the inhibition. The results showed that when the concentration of SDS in the reaction system was greater than or equal to 4.8 × 10-4g / mL, the PCR reaction could be completely inhibited. When the volume ratio of phenol was greater than or equal to 8.0 × 10-3, the DNA polymerase in the system was completely denatured , PCR reaction was completely inhibited.