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以甜瓜品种弥河银瓜为试材 ,研究了利用甜瓜茎再生植株的适宜培养条件 ,并对组培苗的移栽及生产性状进行了对比实验。结果表明 :外植体在MS +BA 1 0～ 1 5mg/L +IBA 0 2mg/L的培养基上获得了最高的不定芽再生率。不定芽经 5 0mg/LIBA预处理 1h后再接种于 12 MS +IAA 0 2mg/L的培养基上 ,生根率达10 0 % ,移栽成活率达 90 %以上。组培苗的生产性状好于种子苗
With the melon variety Mihe melon as test material, suitable culture conditions for regenerating plantlets from melon stems were studied, and the transplanting and production traits of tissue culture seedlings were compared. The results showed that the highest rate of adventitious bud regeneration was obtained on explants with MS + BA 10 ~ 15mg / L and IBA 0 2mg / L. Adventitious buds were pretreated with 50 mg / L IBA for 1 h and then inoculated on 12 MS + IAA 0 2 mg / L medium. The rooting rate reached 100% and the transplanting survival rate was over 90%. Tissue culture seedling production traits better than seedlings