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[目的]观察氯化镉对正常大鼠肾成纤维(NRK)细胞凋亡的形态学及细胞凋亡率的影响。[方法]用20μmol/L氯化镉染毒NRK细胞24h,通过透射电镜观察细胞凋亡形态变化。分别用0、5、10、20、40、60μmol/L的氯化镉培养NRK细胞24h,用20μmol/L的氯化镉分别体外培养NRK细胞0.5、2、6、12、24h,通过流式细胞仪检测各组NRK细胞的凋亡率。[结果]氯化镉可以使NRK细胞出现凋亡征象即凋亡小体的形成,且凋亡率呈时间-效应、剂量-效应关系,剂量为0、5、10、20、40、60μmol/L的氯化镉染毒24h,凋亡率分别为3.01%、6.14%、10.0%、12.6%、23.5%和34.7%;20μmol/l氯化镉染毒NRK细胞0、0.5、2、6、12、24h,凋亡率分别为3.31%、5.96%、8.38%、8.59%、13.97%和16.16%。[结论]氯化镉可以诱导NRK细胞发生特征性的凋亡形态的变化,且氯化镉诱导NRK细胞发生凋亡存在时间-效应、剂量-效应关系。
[Objective] To observe the effect of cadmium chloride on the morphological and apoptotic rate of normal rat kidney fibroblast (NRK) cells. [Method] NRK cells were exposed to 20μmol / L cadmium chloride for 24 hours, and the morphological changes of apoptosis were observed by transmission electron microscope. NRK cells were cultured with 0, 5, 10, 20, 40 and 60μmol / L cadmium chloride respectively for 24 hours. NRK cells were cultured in vitro with 20μmol / L cadmium chloride for 0.5,2,6,12 and 24 hours respectively. Cytometry was used to detect the apoptosis rate of NRK cells in each group. [Results] CdCl2 could induce apoptosis of NRK cells, ie, apoptotic bodies. The apoptosis rate showed a time-effect and dose-response relationship. The dose of 0,5,10,20,40 and 60μmol / L cadmium chloride exposure 24h, the apoptotic rates were 3.01%, 6.14%, 10.0%, 12.6%, 23.5% and 34.7%, respectively; NRK cells were exposed to 20μmol / At 12 and 24 hours, the apoptotic rates were 3.31%, 5.96%, 8.38%, 8.59%, 13.97% and 16.16% respectively. [Conclusion] Cadmium chloride can induce the characteristic morphological changes of NRK cells. There is a time-effect and dose-effect relationship between cadmium chloride and apoptosis of NRK cells.