论文部分内容阅读
目的:探讨参附注射液(SFI)对缺氧缺血性脑损伤(HIBD)新生大鼠eIF2α(p)、ATF4的影响。方法:参照Rice法制备新生大鼠HIBD模型,将7日龄新生SD大鼠随机分为假手术组(S)、生理盐水对照组(C)和参附治疗组(SF),每组按术后观察时间点不同进一步分为HI后6、12、24和72 h 4个亚组。用免疫组织化学方法检测病变侧大脑皮质eIF2α(p)、ATF4水平。结果:C组和SF组eIF2α(p)、ATF4表达水平均较S组升高,差异有统计学意义(P<0.01)。eIF2α(p)、ATF4在HI后12 h达到高峰。HI后6、12、24、72 h SF组eIF2α(p)、ATF4表达水平均低于相应时间点的C组,差异有统计学意义(P<0.01)。结论:HIBD后大鼠病变侧大脑皮质eIF2α(p)、ATF4水平明显升高,SFI下调HIBD新生大鼠eIF2α(p)、ATF4的水平,SFI对新生大鼠HIBD有保护作用。
Objective: To investigate the effect of Shenfu Injection (SFI) on eIF2α (p) and ATF4 in neonatal rats with hypoxic-ischemic brain damage (HIBD). Methods: Neonatal rat model of HIBD was established by reference to Rice method. Seven-day-old SD rats were randomly divided into sham operation group (S), saline control group (C) and Shenfu treatment group (SF) After the observation point of time is further divided into HI after 6,12,24 and 72 h 4 subgroups. Immunohistochemistry was used to detect eIF2α (p), ATF4 levels in the lesion side of cerebral cortex. Results: The expression levels of eIF2α (p) and ATF4 in group C and group SF were higher than those in group S, the difference was statistically significant (P <0.01). eIF2α (p), ATF4 peaked at 12 h after HI. The levels of eIF2α (p) and ATF4 in SF group were lower than those in group C at 6, 12, 24 and 72 h after HI (P <0.01). CONCLUSION: The levels of eIF2α (p) and ATF4 in the cerebral cortex of HIBD rats were significantly increased after HIBD. SFI reduced the levels of eIF2α (p) and ATF4 in neonatal rats with HIBD, while SFI had protective effect on HIBD in neonatal rats.