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目的:观察急性血栓性肺栓塞(acute pulmonary thromboembolism,PTE)兔血清中肝细胞生长因子(hepatocyte growth factor,HGF)的变化特征,并探讨其对PTE的早期诊断价值。方法:新西兰纯种白兔16只,随机均分为对照组和模型组;模型组采用自体血栓回输法建立PTE模型,对照组以等量0.9%氯化钠溶液代替。分别在0、1、3、6、12、24、48、72h采集血样,ELISA法检测HGF及IL-1β含量。结果:模型组血HGF水平于1h后开始升高[(0.853±0.317)μg/L],持续升高到3h略降低后又继续升高,12h升高最明显[(1.742±0.487)μg/L],在48h仍保持高水平,72h开始回落;对照组各时间点血HGF水平均无明显变化,一直保持在较低水平;模型组在1、3、6、12、24、48、72h血清HGF水平均显著高于对照组(P<0.05～0.01)。模型组血IL-1β于0h后开始升高,在3h达到峰值(0.181μg/L),6h降至较低水平(0.09μg/L),直至72h无明显变化;对照组始终在<0.089μg/L水平。结论:HGF有望作为一种新的生物学标记物用于PTE早期诊断。
Objective: To observe the changes of hepatocyte growth factor (HGF) in acute pulmonary thromboembolism (PTE) rabbit serum and to explore its early diagnostic value of PTE. Methods: Sixteen New Zealand white rabbits were randomly divided into control group and model group. PTE model was established by autologous thrombosis in the model group and 0.9% sodium chloride solution in the control group. Blood samples were collected at 0, 1, 3, 6, 12, 24, 48 and 72 h respectively, and the levels of HGF and IL-1β were detected by ELISA. Results: The level of blood HGF in model group began to increase after 1h [(0.853 ± 0.317) μg / L], continued to increase slightly to 3h and then increased slightly, and reached the highest level at 12h [(1.742 ± 0.487) μg / L], remained high at 48h and began to decline at 72h. The levels of HGF in the control group had no significant changes at all time points, and remained at a low level. In the model group at 1,3,6,12,24,48,72h Serum HGF levels were significantly higher than the control group (P <0.05 ~ 0.01). The level of IL-1β in the model group began to increase at 0h, reached the peak at 3h (0.181μg / L) and decreased to the lower level at 6h (0.09μg / L) / L level. Conclusion: HGF is expected to be a new biomarker for the early diagnosis of PTE.