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目的:应用双向凝胶电泳技术(2-DE),分析探讨急性大强度力竭运动后大鼠骨骼肌组织蛋白质组的表达变化及其意义。方法:10只雄性SD大鼠按体重配对随机分为安静组(5只)和运动组(5只)。运动组大鼠按Bedford模型运动至力竭。力竭后3小时处死大鼠,取其后肢腓肠肌全蛋白质进行SDS-固相pH梯度聚丙烯酰胺双向凝胶电泳,运用Bio-radPDquest图像分析软件对2-DE图谱进行分析。结果:安静组显示667±35个蛋白质点,运动组显示572±28个蛋白质点,蛋白质点的匹配率为75%;运动后消失88个蛋白质点,新增32个蛋白质点;运动后蛋白质量上调2倍以上的蛋白质点有12个,蛋白质量下调至1/2以下的蛋白质点有10个,共有282个蛋白质点表达有差异。结论:大鼠大强度力竭运动后3小时,腓肠肌蛋白质发生了明显的质和量的变化。
OBJECTIVE: To study the changes and significance of proteomics in skeletal muscle tissue of rats after acute exhaustive exercise using two-dimensional gel electrophoresis (2-DE). Methods: Ten male Sprague-Dawley rats were randomly divided into 5 groups according to body weight and exercise group (n = 5). Exercise group Rats were exhausted by the Bedford model. Three hours after exhaustion, rats were sacrificed, and the whole gastrocnemius muscle of the hind limbs was subjected to SDS-PAGE and polyacrylamide gel electrophoresis. Bio-radPDquest image analysis software was used to analyze the 2-DE pattern. Results: There were 667 ± 35 protein spots in the quiet group, 572 ± 28 protein spots in the exercise group and 75% in the protein spots. After the exercise, 88 protein spots disappeared and 32 new spots were added. After exercise, There were 12 protein spots up-regulated more than 2 times, 10 protein spots down to less than 1/2 protein spots, and a total of 282 protein spots were expressed differently. CONCLUSION: Three hours after exhaustive exercise, rats’ gastrocnemius muscle had obvious quality and quantity changes.