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以苹果属抗旱能力较强的八棱海棠(Malus robusta Rehd.)和较弱的平邑甜茶[Malus hupehensis(Pamp.)Rehd.]水培幼苗为试验材料,利用细胞形态学、DNAladder等技术,用20%PEG 6000模拟水分胁迫进行诱导细胞程序性死亡研究。经DAPI染色后荧光显微镜观察发现两个苹果属材料均呈现细胞染色质凝集、细胞核缩小、变形、解体等细胞程序性死亡的形态学特征;同时,DNA琼脂糖凝胶电泳可观察到明显的“梯状”DNA条带(DNA ladder)。由此表明,水分胁迫诱导苹果属植物具有明显的细胞程序性死亡的典型形态及生化特征,是一种主动的细胞死亡过程,八棱海棠细胞程序性死亡持续的时间长于平邑甜茶。
Malus robusta Rehd. And Weak Malus hupehensis (Pamp.) Rehd., A hydroponic seedling of Malus robusta (Pamp.) Rehd., With strong drought tolerance of apple genus, were used as experimental materials. Cell morphological and DNAladder techniques, Study on Induction of Programmed Cell Death Using 20% PEG 6000 Simulated Water Stress. After DAPI staining, it was observed by fluorescence microscopy that morphological features of apoptotic cell apoptosis such as chromatin condensation, nuclear shrinkage, deformation and disintegration were observed in both apple genus materials. At the same time, DNA agarose gel electrophoresis showed obvious “Ladder” DNA ladder. The results showed that water stress induced the typical morphological and biochemical characteristics of apple plant, which was an active process of cell death. Proliferation of begonias induced a longer duration of apoptosis than that of Pingyi sweet tea.