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目的 :探讨头颈部鳞癌的微卫星不稳定性 (MSI)及杂合性丢失 (LOH)。方法 :选择来自 3、5、6、8、9、13、17和 18号染色体的 15个微卫星标志对 36例头颈部鳞癌标本和相应的外周血进行微卫星分析。结果 :36例头颈部鳞癌中 ,2 7.8% (10 / 36 )分别有 1- 8个位点存在MSI,MSI发生率较高的位点为 :D17S5 2 0 (2 2 9% )、D6S10 5 (16 7% )和D8S2 6 4(13 9% )。在 9p2 1-p2 2和 3p14等处存在一定的LOH。微卫星异常的检出率与肿瘤分期、分级无相关性。结论 :提示MSI是头颈部鳞癌中较为常见的遗传学变化 ,染色体 9p2 1-p2 2和 3p14区域可能存在与头颈部鳞癌有关的抑癌基因。
Objective : To investigate the microsatellite instability (MSI) and loss of heterozygosity (LOH) in head and neck squamous cell carcinoma. Methods: Thirty-six head and neck squamous cell carcinoma specimens and corresponding peripheral blood samples were analyzed by microsatellite analysis using 15 microsatellite markers from chromosomes 3, 5, 6, 8, 9, 13, 17 and 18. RESULTS: Of the 36 head and neck squamous cell carcinomas, MSI was present in 2 7.8% (10 / 36) of the lesions in 1- 8 loci, and the highest incidence of MSI was: D17S5 2 0 (22 9%). D6S10 5 (16 7%) and D8S2 6 4 (13 9%). There is a certain amount of LOH at 9p2 1-p2 2 and 3p14. There was no correlation between the detection rate of microsatellite abnormalities and the stage and grade of tumors. Conclusion : It is suggested that MSI is a relatively common genetic change in head and neck squamous cell carcinoma. There may be tumor suppressor genes related to head and neck squamous cell carcinoma in chromosome 9p2 1-p22 and 3p14 regions.