目的分析比较牙周可疑致病菌的核磁共振代谢图谱,探讨用代谢组学方法快速鉴定口腔细菌的可能性。方法分别在BHI液体培养基中接种相同密度的牙龈卟啉单胞菌、中间普氏菌和具核梭杆菌,采用比浊法绘制生长曲线。取3种细菌生长稳定期的培养液进行核磁共振氢谱(1H-NMR)测定,用主成分分析法进行数据分析。结果主成分分析显示3组数据各自有较为集中的类聚关系,可以区分这3种细菌。结论代谢组学是一种具有良好应用前景的口腔细菌分类鉴定方法。 OBJECTIVE: To analyze and compare the nuclear magnetic resonance (MR) maps of suspected periodontal pathogens and to explore the possibility of rapid identification of oral bacteria by metabolomics. Methods The same density of Porphyromonas gingivalis, Prevotella intermedia and Fusobacterium nucleatum were inoculated into BHI broth respectively and the growth curves were drawn by turbidimetry. Three kinds of culture broths were used for 1H-NMR determination of the growth of the bacteria, and the data were analyzed by principal component analysis. The results of principal component analysis showed that the three groups of data each have a more clustering relationship, which can distinguish the three kinds of bacteria. Conclusion Metabolomics is a promising method of oral bacteria classification and identification.