一、灭菌时,高压锅内的空气要排放干净,灭菌压力要达到1.2公斤/平方厘米以上,灭菌时间要足30分钟。斜面制好后放2～3天再进行接种。二、接种前,手要用70%的酒精棉球擦2遍;接种工具要用酒精棉球擦并在酒精灯火焰上烧,交替进行两次。三、在接种以前,要认真检查种子是否纯净,是否老化,不纯净或已老化的种子不能再用于转接。 First, when sterilizing, the air inside the pressure cooker should be drained cleanly, the sterilization pressure should reach above 1.2kg / cm, the sterilization time should be enough for 30 minutes. Bevel system made after 2 to 3 days before inoculation. Second, before inoculation, hand use 70% alcohol cotton ball to wipe 2 times; inoculation tools use alcohol cotton ball rub and burn in the alcohol lamp flame, alternating twice. Third, prior to inoculation, the seeds should be carefully checked for purity, aging, impure or aged seeds can no longer be used for transfer.