目的探讨高效氯氰菊酯对人皮肤表皮细胞的直接刺激损害。方法以体外浸泡式培养的人皮肤表皮细胞为生物材料,以培养基中乳酸脱氢酶(LDH)活性为指标,检测了在不同染毒浓度条件下培养基中的LDH活性,并进行了电镜观察。结果随着染毒剂量的增加,培养基中LDH活性升高,与阴性对照组相比,差异有统计学意义(P<0.05或P<0.01);与阳性对照组相比,差异亦有统计学意义(P<0.01);电镜观察显示,在低浓度条件下,细胞虽可保持结构完整,但表面的绒毛状突起减少,少数细胞胞核内出现空泡(水肿),细胞受到轻微损害。随染毒剂量增加,细胞胞膜破裂,细胞核溶解呈空泡状,乃至细胞大量坏死。结论高效氯氰菊酯对人皮肤表皮细胞有直接刺激损害作用,在一定条件下,可损害皮肤屏障,使其更易被吸收,造成机体中毒,应特别注意劳动防护。 Objective To investigate the direct stimulation of beta-cypermethrin on human skin epidermal cells. Methods Human skin epidermal cells cultured in vitro were used as biomaterials. The activities of LDH in culture medium were measured. The activities of LDH in medium were detected under electron microscope Observed. Results Compared with the negative control group, the LDH activity in the medium increased with the increase of exposure dose (P <0.05 or P <0.01). There was also a statistically significant difference between the two groups (P <0.01). Electron microscopy showed that cells maintained intact structure at low concentration, but there were fewer villous processes on the surface, vacuoles (edema) appeared in the nuclei of a few cells, and the cells were slightly damaged. With the dose increased, the cell membrane rupture, vacuolization nucleolysis, and even a large number of cell necrosis. Conclusion Beta-cypermethrin can directly damage human skin epidermal cells. Under certain conditions, it can damage the skin barrier and make it more easily absorbed, resulting in organism poisoning. Special attention should be paid to labor protection.