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目的 :探讨灯盏花素对鼠脑梗塞面积、神经功能缺失征象及鼠脑缺血边缘区神经细胞凋亡的影响。方法 :采用TTC染色、临床神经功能评分、流式细胞分析和原位末端标记等方法 ,分别对缺血、缺血再灌注及灯盏花素治疗组鼠脑梗塞面积、神经功能缺失征象、鼠脑缺血边缘区神经细胞的凋亡进行观察和比较。结果 :2 4只大鼠缺血 /再灌后TTC染色可见右侧顶叶皮质出现恒定苍白梗塞灶 ,灯盏花素治疗组于相同时间点脑梗塞面积较缺血 /再灌组明显减小 ;缺血 /再灌组大鼠均有明显的神经功能缺失征象 ,而药物治疗组 12只大鼠中只有 3只出现轻度的神经功能缺损。假手术组仅有极少量神经细胞凋亡 ,缺血 90min/再灌 12h、2 4h组缺血边缘区神经细胞凋亡明显增多 ,灯盏花素治疗组于相同时限内细胞凋亡较缺血 /再灌组显著减少 (P <0 .0 1)。结论 :蛋白激酶C抑制剂灯盏花素能明显减小鼠脑缺血后脑梗塞面积 ,减轻脑缺血后神经功能缺损 ,显著减少缺血边缘区神经细胞凋亡数量 ,减缓缺血区神经元损害 ,具有显著的脑保护作用
Objective: To investigate the effects of breviscapine on the area of cerebral infarction, neurological deficits, and neuronal apoptosis in the peripheral cerebral ischemic region of rats. METHODS: TCT staining, clinical neurological function scores, flow cytometric analysis, and in situ end labeling methods were used to measure the cerebral infarction area, neurological deficits, and rat brain damage in the ischemia, ischemia-reperfusion and breviscapine groups, respectively. Apoptosis of neurons in the ischemic marginal zone was observed and compared. RESULTS: Twenty-four rats showed persistent pale infarction in the right parietal cortex after TTC staining. The area of cerebral infarction at the same time point in the breviscapine treatment group was significantly lower than that in the ischemia/reperfusion group. Ischemia / reperfusion group rats had significant signs of neurological deficits, while only 3 of the 12 rats in the drug treatment group had mild neurological deficits. In the sham operation group, there was only a very small amount of neuronal cell apoptosis. Apoptosis of the ischemic marginal neurons was significantly increased in the ischemic 90 min/reperfusion 12 h and 24 h groups. The apoptosis in the breviscapine treatment group was more than ischemic in the same time frame. The reperfusion group was significantly reduced (P < 0.01). CONCLUSION: The protein kinase C inhibitor breviscapine can significantly reduce the cerebral infarct size in mice after cerebral ischemia, reduce the neurological deficit after cerebral ischemia, and significantly reduce the number of neurons apoptosis in the ischemic marginal zone and reduce the neuronal damage in the ischemic area. With significant brain protection