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目的:研究心力衰竭患者血清氨基末端B型钠尿肽原(NT-proBNP)水平与同型半胱氨酸(Hcy)浓度是否具有相关性,并探讨其可能机制.方法:选择因心力衰竭、心功能Ⅲ～Ⅳ级就诊的患者.根据血清Hcy浓度是否≥10μmol/L,将患者分为正常同型半胱氨酸组(NHcy组)及高同型半胱氨酸组(HHcy组).入院检测患者NT-proBNP,比较2组患者基线资料及NT-proBNP水平.同时培养H9C2大鼠心肌细胞,使用CCK-8检测Hcy对心肌细胞的损伤作用,并摸索最适浓度,用最适Hcy浓度干预细胞72 h后行罗氏Tunel染色,评估细胞凋亡.结果:NHcy组与HHcy组基线资料差异无统计学意义(P＞0.05),HHcy组患者血清NT-proBNP平均水平高于NHcy组(P＜0.05).同时细胞模型CCK-8显示:经不同浓度Hcy干预,A值逐渐下降,与对照组比较差异有统计学意义(P＜0.05);罗氏Tunel染色显示:经最适Hcy浓度干预72h后,凋亡明显增加,与对照组比较差异有统计学意义(P＜0.05).结论:心力衰竭患者NT-proBNP水平与血清Hcy浓度具有相关性,HHcy致NT-proBNP增高,可能与其通过介导心肌细胞凋亡引起心肌损伤有关.“,”Objective:To study the correlation between serum NT-proBNP level and HHcy concentration in patients with heart failure,and to explore the possible mechanism.Method:We chose the patients with heart failure in the grade Ⅲ-Ⅳ cardiac function evaluated by NYHA in our hospital from October 2015 to April 2016.The patients were divided into HHcy group and NHcy group according to whether the serum Hcy concentration was greater than 10 μmol/L.NT-proBNP concentrations were detected.H9C2 rat myocardial cells were cultured,At the same time the injury of Hcy on H9C2 cells were detected by CCK-8.We chose the optimal concentration of Hcy to stimulate cells.After 72 hours,Tunel staining was performed to evaluate the apoptosis of the cells.Result:There was no significant difference between the two groups in baseline data(P＞0.05).However,the average level of serum NT-proBNP in patients with HHcy were higher than that in group NHcy (P＜0.05).At the same time,the CCK-8 of the cell model showed that the OD values decreased after treatment with Hcy in dose-dependent (P＜0.05).Tunel staining showed the apoptosis of the H9C2 cells were significantly increased after treatment with Hcy (P＜0.05).Conclusion:Serum levels of Hcy is associated to NT-proBNP in patients with heart failure.