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目的探讨微小RNA(miR)-340-3p在骨髓增生异常综合征(MDS)患者骨髓中的表达情况,并应用生物信息学技术分析其靶基因及功能。方法利用基因表达谱芯片结合分层聚类在9例MDS患者和6例健康正常人骨髓中筛选出差异表达的miR-340-3p,并利用miRBase和Miranda软件预测其靶基因,并对预测的靶基因进行功能富集和信号通路分析。结果与健康正常人比较,miR-340-3p在MDS患者骨髓中表达下调(P<0.05)。获得共同76个潜在靶基因,包括BANP、KIF2C、DIDO1、GRM1等。基因本体(GO)分析显示靶基因主要富集于细胞生物学、分子功能、细胞组分,信号通路分析显示其主要富集于FoxO信号通路、NOD样受体信号通路、造血细胞系、Gap连接等信号转导通路等。结论 miR-340-3p在MDS患者骨髓中表达下调,其可能通过靶向负向调控下游的靶基因而参与MDS的发生发展。
Objective To investigate the expression of microRNA (miR) -340-3p in the bone marrow of patients with myelodysplastic syndrome (MDS) and analyze the target genes and their functions by using bioinformatics techniques. Methods The differentially expressed miR-340-3p was screened from 9 patients with MDS and 6 healthy controls using gene expression microarray and hierarchical clustering. The target genes were predicted by miRBase and Miranda software. Target genes for functional enrichment and signal pathway analysis. Results Compared with healthy controls, miR-340-3p was down-regulated in the bone marrow of MDS patients (P <0.05). A total of 76 potential target genes were obtained, including BANP, KIF2C, DIDO1 and GRM1. Gene Ontology (GO) analysis showed that the target genes were mainly enriched in cell biology, molecular function, cell components, and signal pathway analysis showed that they were mainly enriched in FoxO signaling pathway, NOD-like receptor signaling pathway, hematopoietic cell line, Gap junction Such as signal transduction pathway. Conclusion The expression of miR-340-3p is down-regulated in the bone marrow of patients with MDS, which may be involved in the development of MDS by targeting downstream target genes negatively.