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目的 通过检测细胞周期素依赖激酶抑制蛋白p2 7Kip1(p2 7)和增殖细胞核抗原Ki 67在肾透明细胞癌中的表达情况 ,探讨其与临床病理指标及预后的关系。方法 采用免疫组化SP法测定 42例肾透明细胞癌及 2 0例癌旁正常肾组织中p2 7蛋白和Ki 67抗原的表达。结果 在肾透明细胞癌与癌旁正常肾组织中p2 7或Ki 67的表达均有显著性差异(P <0 .0 5 )。肾透明细胞癌的病理分级与p2 7的表达呈负相关 ,而与Ki 67抗原的表达呈正相关 (P <0 .0 1)。肾透明细胞癌中p2 7表达与Ki 67抗原表达、肿瘤有无转移呈负相关 (P <0 .0 1) ;Ki 67抗原表达与肿瘤有无转移呈正相关 (P <0 .0 1)。结论 在人肾透明细胞癌的发生、发展及转移过程中 ,由于缺乏p2 7基因持续激活的后继支撑 ,p2 7蛋白被逐渐消耗 ,Ki 67抗原表达上调 ;p2 7表达与Ki 67表达对评估肾透明细胞癌患者预后有一定价值。
Objective To investigate the relationship between the expression of cyclin-dependent kinase inhibitor protein p2 7Kip1 (p27) and proliferating cell nuclear antigen Ki 67 in clear cell renal cell carcinoma (RCCC) and its relationship with clinicopathological factors and prognosis. Methods Immunohistochemical SP method was used to detect the expression of p27 and Ki67 in 42 cases of clear cell renal cell carcinoma and 20 cases of adjacent normal renal tissues. Results There was a significant difference in the expression of p27 or Ki67 between renal clear cell carcinoma and adjacent normal renal tissue (P <0.05). The pathological grade of renal clear cell carcinoma was negatively correlated with the expression of p27, but positively correlated with the expression of Ki 67 antigen (P <0.01). There was a negative correlation between the expression of p27 in clear cell renal cell carcinoma and the expression of Ki67 antigen and the presence or absence of metastasis (P <0.01). The positive expression of Ki 67 antigen was correlated with the metastasis of tumor (P <0.01). Conclusions In the process of the occurrence, development and metastasis of human clear cell carcinoma, the p27 protein is gradually depleted due to the lack of sustained support of p2 7 gene, and the expression of Ki 67 antigen is up-regulated. The expression of p27 and Ki 67 is positively correlated with The prognosis of patients with clear cell carcinoma has some value.