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以实验室筛选的溶磷能力最强的磷细菌W37为试验菌株,采煤沉陷区复垦土壤为供试土样,通过盆栽试验研究施用磷细菌对复垦土壤磷素养分、油菜产量、磷吸附特性及流失风险的影响。结果表明:磷细菌可以提高复垦土壤有效磷(Olsen-P)含量和油菜产量;施用磷细菌后复垦土壤最大吸磷量(Xm),吸附常数(k)都减小;磷细菌处理易解吸磷(RDP)、磷零点吸持平衡浓度(EPC0)及磷素饱和度(DPS)呈增加的趋势,分别比M处理增加0.014mg·L~(-1),6.54ug·L~(-1),0.80%;通过估算复垦土壤Olsen-P、EPC0、DPS的环境风险值为28.52mg·kg~(-1),129.78ug·L~(-1),5.18%,本试验中磷细菌处理土壤Olsen-P、EPC0、DPS远小于风险值,因此复垦土壤上施用磷细菌对磷素流失风险影响较小。
Phosphate bacteria W37 with the strongest phosphate solubilizing ability in laboratory was used as test strain. The reclaimed soil in subsidence area of mining coal was used as test soil sample. Pot experiment was conducted to study the effects of phosphorus bacteria on reclaimed soil phosphorus, Adsorption characteristics and the risk of loss. The results showed that: phosphorus bacteria could increase the contents of available phosphorus and the yield of rape in reclaimed soil; the maximum phosphorus uptake (Xm) and the adsorption constant (k) of reclaimed soil decreased after phosphorus bacteria were used; The concentrations of RDP, EPC0 and PPS tended to increase, which were respectively increased by 0.014mg · L -1 and 6.54ug · L ~ (-1) than M treatment, 1) and 0.80% respectively. The environmental risk of EPC0 and DPS was estimated to be 28.52 mg · kg -1, 129.78 μg · L -1 and 5.18% respectively by estimating Olsen-P in reclaimed soil. In this experiment, Bacterial treatment of soil Olsen-P, EPC0, DPS far less than the risk value, so the phosphorus bacteria on reclaimed soil phosphorus release risk less affected.