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目的建立一个小鼠无精子症模型,并探讨Mm.158494基因在无精子症中的表达及意义。方法建立无精子症小鼠模型,观察小鼠睾丸生精小管结构的变化。筛选与精子发生相关的睾丸特异性新基因,利用网络信息资源对该基因进行生物学信息分析,RT-PCR分析该基因在无精子症小鼠睾丸中的表达。结果生物信息学分析发现Mm.158494基因cDNA序列全长1046bp,含有762bp的完整开放阅读框。编码253个氨基酸、分子量为29.432kDa的蛋白质。小鼠无精子症模型中微弱表达Mm.158494基因。结论Mm.158494基因在无精子症中表达明显降低,提示该基因可能在精子发生中起重要作用。
Objective To establish a murine model of azoospermia and investigate the expression and significance of Mm.158494 in azoospermia. Methods Azoospermia mouse model was established to observe the changes of seminiferous tubule structure in mouse testes. The new testis-specific genes related to spermatogenesis were screened. The biological information of the gene was analyzed by internet information resources. The expression of the gene in the testes of azoospermic mice was analyzed by RT-PCR. Results Bioinformatics analysis revealed that the Mm.158494 gene cDNA sequence was 1046 bp in length and contained a 762 bp complete open reading frame. A protein encoding 253 amino acids with a molecular weight of 29.432 kDa. Mouse azoospermia model weakly expresses Mm.158494 gene. Conclusion Mm.158494 gene expression in azoospermia significantly reduced, suggesting that the gene may play an important role in spermatogenesis.